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hiscript ii reverse transcriptase  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript ii reverse transcriptase
    Hiscript Ii Reverse Transcriptase, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase/HiScript+II+Reverse+Transcriptase/custom%40r201%4042495521
    Average 97 stars, based on 1479 article reviews
    hiscript ii reverse transcriptase - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Identification of a Novel Glycosyl Transferase Family 17 Protein Involved in Cd Accumulation in Rice (Oryza sativa).
    Article Snippet: Cadmium (Cd) is a heavy metal widely distributed in the environment that poses a significant threat to living organisms because of its strong mobility and toxicity.. In this study, a novel gene named Osßglu from the glycosyltransferase (GT) family was investigated for its role in the Cd stress response in rice.. Various experiments were conducted using the japonica cultivar Nipponbare (Nip) and its mutants (Osßglu1 and Osßglu2).

    Article Title: Caspase-11 deficiency ameliorates elastase-induced abdominal aortic aneurysm in mice by suppressing inflammatory response of macrophages.
    Article Snippet: 171 RNA extraction and real-time PCR 172 Total RNA was extracted from frozen aortic tissue or cultured cells using Trizol reagent 173 (Thermo Fisher Scientific), followed by chloroform extraction according to the 174 manufacturer’s instruction. .. Aliquots of total RNA were reverse transcribed into single-175 stranded cDNA by incubation with reverse transcriptase (Vazyme). .. Real-time PCR was 176 performed with SYBR Premix Taq kits (Vazyme) according to the manufacturer’s 177 Downloaded from journals.physiology.org/journal/ajpcell (2A09:BAC5:3A22:25CD:0000:0000:03C4:0011) on June 7, 2025.

    Article Title: Tet2 Protects Against Isoniazid‐Induced Hepatotoxicity via Regulating Autophagy
    Article Snippet: Hepatotoxicity is a significant clinical challenge that severely limits the therapeutic application of isoniazid (INH), a globally recommended firstline antituberculosis (TB) drug.. Despite its essential role in TB treatment, the underlying mechanisms of INHinduced liver injury remain incompletely understood.. This study investigates the role of Teneleven translocation 2 (Tet2) in INHinduced hepatotoxicity and elucidates its regulatory mechanism on autophagy during hepatocellular injury.

    Article Title: Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L.
    Article Snippet: Ferroptosis is an important cause of cardiomyocyte loss and cardiac dysfunction.. Cathelicidin-related antimicrobial peptide (CRAMP) is an endogenous polypeptide that regulates oxidative stress in the body and is involved in ferroptosis.. However, its specific role and mechanism in ferroptosis are unclear.

    Article Title: Senescent cells promote viral infection-associated inflammation and tissue damage through a robust NF-κB pathway
    Article Snippet: .. The Reverse transcription for cDNA synthesis was performed using 1 μg of RNA by reverse transcriptase (Vazyme Biotech Co., Ltd., China). ..

    Article Title: Mediator complex subunit MED23 dampens antiviral innate immunity by restricting RIG-I expression
    Article Snippet: .. Total RNA was extracted from mouse tissues or cells using TRIzol reagent (Invitrogen) and reverse transcribed with reverse transcriptase (Vazyme). .. The resulting cDNA samples were amplified by quantitative PCR with SYBR Green Realtime PCR Master Mix (Vazyme) and gene-specific primers using a Life QuantStudio7 Flex Real-Time PCR System.

    Article Title: Senescent cells promote viral infection-associated inflammation and tissue damage through a robust NF-κB pathway.
    Article Snippet: .. The Reverse transcription for cDNA synthesis was performed using 1 μg of RNA by reverse transcriptase (Vazyme Biotech Co., Ltd., China). ..

    Article Title: OsSSID6 Negatively Regulates Salt Stress Tolerance in Rice ( Oryza Sativa L.) via Metabolic Pathways and ROS Scavenging
    Article Snippet: Total RNA extraction from all samples was performed using Trizol reagent (Nanjing Vazyme Biotechnology Co., Ltd., China). .. Subsequently, cDNA was synthesized by reverse transcriptase (Nanjing Vazyme Biotechnology Co., Ltd., China) and was used for RT-qPCR, using a SYBR GreenPCR Master Mix kit (ABclonal Biotechnology Co., Ltd., China), to analyze the transcriptional accumulation of target genes. ..

    cDNA Synthesis:

    Article Title: Identification of a Novel Glycosyl Transferase Family 17 Protein Involved in Cd Accumulation in Rice (Oryza sativa).
    Article Snippet: Cadmium (Cd) is a heavy metal widely distributed in the environment that poses a significant threat to living organisms because of its strong mobility and toxicity.. In this study, a novel gene named Osßglu from the glycosyltransferase (GT) family was investigated for its role in the Cd stress response in rice.. Various experiments were conducted using the japonica cultivar Nipponbare (Nip) and its mutants (Osßglu1 and Osßglu2).

    Article Title: Senescent cells promote viral infection-associated inflammation and tissue damage through a robust NF-κB pathway
    Article Snippet: .. The Reverse transcription for cDNA synthesis was performed using 1 μg of RNA by reverse transcriptase (Vazyme Biotech Co., Ltd., China). ..

    Article Title: Senescent cells promote viral infection-associated inflammation and tissue damage through a robust NF-κB pathway.
    Article Snippet: .. The Reverse transcription for cDNA synthesis was performed using 1 μg of RNA by reverse transcriptase (Vazyme Biotech Co., Ltd., China). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of a Novel Glycosyl Transferase Family 17 Protein Involved in Cd Accumulation in Rice (Oryza sativa).
    Article Snippet: Cadmium (Cd) is a heavy metal widely distributed in the environment that poses a significant threat to living organisms because of its strong mobility and toxicity.. In this study, a novel gene named Osßglu from the glycosyltransferase (GT) family was investigated for its role in the Cd stress response in rice.. Various experiments were conducted using the japonica cultivar Nipponbare (Nip) and its mutants (Osßglu1 and Osßglu2).

    Article Title: Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L.
    Article Snippet: Ferroptosis is an important cause of cardiomyocyte loss and cardiac dysfunction.. Cathelicidin-related antimicrobial peptide (CRAMP) is an endogenous polypeptide that regulates oxidative stress in the body and is involved in ferroptosis.. However, its specific role and mechanism in ferroptosis are unclear.

    Incubation:

    Article Title: Caspase-11 deficiency ameliorates elastase-induced abdominal aortic aneurysm in mice by suppressing inflammatory response of macrophages.
    Article Snippet: 171 RNA extraction and real-time PCR 172 Total RNA was extracted from frozen aortic tissue or cultured cells using Trizol reagent 173 (Thermo Fisher Scientific), followed by chloroform extraction according to the 174 manufacturer’s instruction. .. Aliquots of total RNA were reverse transcribed into single-175 stranded cDNA by incubation with reverse transcriptase (Vazyme). .. Real-time PCR was 176 performed with SYBR Premix Taq kits (Vazyme) according to the manufacturer’s 177 Downloaded from journals.physiology.org/journal/ajpcell (2A09:BAC5:3A22:25CD:0000:0000:03C4:0011) on June 7, 2025.

    Isolation:

    Article Title: Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L.
    Article Snippet: Ferroptosis is an important cause of cardiomyocyte loss and cardiac dysfunction.. Cathelicidin-related antimicrobial peptide (CRAMP) is an endogenous polypeptide that regulates oxidative stress in the body and is involved in ferroptosis.. However, its specific role and mechanism in ferroptosis are unclear.

    Synthesized:

    Article Title: OsSSID6 Negatively Regulates Salt Stress Tolerance in Rice ( Oryza Sativa L.) via Metabolic Pathways and ROS Scavenging
    Article Snippet: Total RNA extraction from all samples was performed using Trizol reagent (Nanjing Vazyme Biotechnology Co., Ltd., China). .. Subsequently, cDNA was synthesized by reverse transcriptase (Nanjing Vazyme Biotechnology Co., Ltd., China) and was used for RT-qPCR, using a SYBR GreenPCR Master Mix kit (ABclonal Biotechnology Co., Ltd., China), to analyze the transcriptional accumulation of target genes. ..



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